Mitochondrial DNA Isolation

What’s mitochondrial DNA? mitochondrial DNA (mtDNA) is a genetic material of the mitochondria, the organelles that generate energy for the cell. In mammals, mtDNA makes up less than 1% of the total cellular DNA, but in plants the amount is variable. It codes for ribosomal RNA and transfer RNA, but only some mitochondrial proteins (up to 30 proteins in animals), the nuclear DNA being required for encoding most of these. Mitochondrial DNA which is passed down from the mother to all her children, males and females

Protocol 1. Mitochondrial DNA Isolation (BioVision)

Protocol 2. Mitochondrial DNA Isolation

Protocol 3. Comparison of different methods for extraction of mitochondrial …

quality of DNA. Some reports have described obtaining mtDNA. using techniques based on the preparation of spheroplastis or. isolation of mitochondria (6,15 …
www.nih.go.jp/JJID/55/122.pdf

Protocol 4. Purification of mitochondrial DNA.

A minimum of 2-3 g wet weight of cells are necessary for one DNA extraction (preferably 10-20 g). The cells are harvested in the early stationary phase by …
www.bch.umontreal.ca/People/lang/FMGP/methods/mtDNA.html

Protocol 5. Fossil Hominids: mitochondrial DNA

1997) claimed to have extracted mitochondrial DNA (mtDNA) from a piece of bone cut from the upper arm of the first recognised Neandertal fossil, …
www.talkorigins.org/faqs/homs/mtDNA.html

Protocol 6. Mitochondrial DNA Analysis at the FBI Laboratory

  • Backgroung of mitochondril DNA
  • Current problems
  • Test procedures
  • Database
  • Other fosenic information related to mitochondrial DNA

 

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