Protocol for Zymogram

DO NOT HEAT)

4- Load samples directly onto gel

5- Run gel

6- Wash 2X 20min in wash buffer

7- Wash 10min in incubation buffer

8- Place gel in sealable container with fresh incubation buffer and incubate

at 37ºC for 24h to 48h

9- Fix and stain with fresh Coomassie Blue solution

10-Destain with MeOH:AcOH:H2O(5:1:5)

11-Replace with 10% AcOH and continue destaining

12-Photpgraph and dry gel for storage

 

(Lee, Sunyoung)

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