Probing RNA Folding Pathways by RNA Fingerprinting

Probing RNA Folding Pathways by RNA Fingerprinting

SA Woodson. Curr Protoc Nucleic Acid Chem 70, 11.4.1-11.4.19. 2017 Sep 18.

Abstract

This unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or seconds, and that are thus difficult to study by solution-based methods. Conformers that have been separated and immobilized in the gel matrix can be used to study catalytic activity with or without being eluted from the gel. The method can be applied to a wide variety of catalytic RNAs and RNA-protein complexes. © 2017 by John Wiley & Sons, Inc.

Copyright © 2017 John Wiley & Sons, Inc.

PubMed: 28921495

DOI: 10.1002/cpnc.36

 

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