Biorptocol: DNA Modification/Epigenetics

Analysis of Mitotic Checkpoint Function in Xenopus Egg Extracts

Yinghui Mao

Cold Spring Harb Protoc 2018; doi:10.1101/pdb.prot099853

Abstract  Full Text (PDF)

 

Analysis of DNA Methylation in Mammalian Cells

Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee

Cold Spring Harb Protoc 2017; doi:10.1101/pdb.top094821

Abstract  Full Text  Full Text (PDF)

 

Methylation-Specific Polymerase Chain Reaction (PCR) for Gene-Specific DNA Methylation Detection Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee

Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot094847

Abstract  Full Text  Full Text (PDF)

 

Methyl-Cytosine-Based Immunoprecipitation for DNA Methylation Analysis

Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee

Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot094854

Abstract  Full Text  Full Text (PDF)

 

High-Throughput Deep Sequencing for Mapping Mammalian DNA Methylation

Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee

Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot094862

Abstract  Full Text  Full Text (PDF)

 

DNA Bisulfite Sequencing for Single-Nucleotide-Resolution DNA Methylation Detection

Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee

Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot094839

Abstract  Full Text  Full Text (PDF)

 

Illumina Sequencing of Bisulfite-Converted DNA Libraries

Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee

Cold Spring Harb Protoc 2017; doi:10.1101/pdb.prot094870

Abstract  Full Text  Full Text (PDF)

 

Micrococcal Nuclease Digestion of Schizosaccharomyces pombe Chromatin

Hugh P. Cam and Simon Whitehall

Cold Spring Harb Protoc 2016; doi:10.1101/pdb.prot091538

Abstract  Full Text  Full Text (PDF)

 

Oncogenomics Methods and Resources

Simon J. Furney, Gunes Gundem, and Nuria Lopez-Bigas

Cold Spring Harb Protoc 2012; doi:10.1101/pdb.top069229

Abstract  Full Text  Full Text (PDF)

 

Detection of Cytosine Methylation in RNA Using Bisulfite Sequencing

Tim Pollex, Katharina Hanna, and Matthias Schaefer

Cold Spring Harb Protoc 2010; doi:10.1101/pdb.prot5505

Abstract  Full Text  Full Text (PDF)

 

In Vitro Histone Demethylase Assay

Yu-ichi Tsukada and Keiichi I. Nakayama

Cold Spring Harb Protoc 2010; doi:10.1101/pdb.prot5512

Abstract  Full Text  Full Text (PDF)

 

Potassium Permanganate Probing of Pol II Open Complexes

Michael F. Carey, Craig L. Peterson, and Stephen T. Smale

Cold Spring Harb Protoc 2010; doi:10.1101/pdb.prot5479

Abstract  Full Text  Full Text (PDF)

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