- Weigh out 60 mg ATP.
- Add 0.8 ml distilled water.
- Adjust to pH 7.0 with 0.1 M NaOH.
- Make up volume to 1 ml with distilled water.
<STORAGE> Aliquots of appropriate volume should be stored at -70 C.
10 M Ammonium acetate (1 liter)
- Weigh out 770 g ammonium acetate.
- Add 800 ml distilled water to dissolve.
- Make up volume to 1 liter with distilled water.
- Sterilize by filtration.
<CRITICAL> Ammonium acetate is heat labile. Do not autoclave.
<STORAGE> Store at 4 C.